These are the relationships Anais has with the other characters in the series.
Family
Gumball
Gumball and Anais are making up and hugging.
Gumball is Anais' older brother. She finds him foolish and stupid. She doesn't usually act or seem as if she loves her brother. Though she may seem to think very poorly of him, she does have her moments when she admits she loves him or cares for him, and he is willing to go to extreme lengths to help her out such as trying to retrieve her Daisy doll in "The Quest." In terms of maturity, she is the "older sister," but she does look up to him. She can sometimes be very angry or cross with him whenever he does something wrong (even if it is accidental), and when he was the babysitter, she decided she could do whatever she wanted. Despite her rude snarks and occasionally bratty remarks towards him, she still loves her older brother.
Darwin
Darwin and Anais are close siblings.
Darwin is Anais' older, adopted brother. She doesn't seem to think Darwin is as stupid as Gumball. They seem to get along well and can be seen together at school many times. Anais likes Darwin more than Gumball as seen first in "The Goons," since she gets along better with him when she decided to act like a dumb person. Anais has shown that she loves, and cares for Darwin as well. Also, they always team up to do something important, whether it be stopping Gumball from using the Lucky Helmet or Anais doing Darwin's aptitude test to help him. In "The Roots," she is heartbroken when she (and the rest of the Wattersons) decide that they have to let Darwin back into the wild. In the same episode, it is revealed that Darwin is her favorite brother.
Nicole
Mary, Nicole, and Anais on an outing for Nicole's birthday.
As the mother of Anais, Nicole loves her youngest and only daughter. She is very protective of her and only wants her to be safe and happy. Nicole recognizes Anais' intelligence and often wants to solve puzzles with her, taking pictures of the finished piece as seen in "The Goons." Although they enjoy spending time with one another, Anais usually gets frightened when Nicole becomes furious.
Richard
Anais and her Dad.
Richard cares about his daughter's well-being, going so far as to get a job for Anais despite his intense hatred of working as shown in "The Painting." Richard tries to comfort her as shown in "The Pony" by saying the right thing, even though he fails and tells her to speak to her mother. Richard also attempts to protect Anais when she's scared in "The Vacation".
Granny Jojo
Anais helps Granny Jojo meet up with Louie.
Anais resembles her grandmother greatly. In "The Kiss," she struggles to get Granny Jojo's heavy luggage upstairs. After spending a night finishing this task, she gets angry when she is asked to bring it down again. In "The Authority," Granny Jojo keeps her safe, and protects her (at the cost of Anais' intelligence). In "The Man," Anais helps Granny Jojo meet up with Louie.
Louie
Louie gives the check as a gift for being his favorite grandchildren.
In The Check, when Louie is later married to Granny Jojo and becomes the Watterson children's step-grandfather, Louie gives a check of $50 (that the children initially believed to be $5000) as a gift for "being his favorite grandchildren."
Failed Friendship Attempts
Chimera
Chimera and Anais were each other's first true friends.
In "The Friend," Anais becomes friends with Chimera, though the friendship started off somewhat awkward. When the Chimera is revealed to be a criminal, Anais and her brothers defend him from the government. She was very upset when he exploded, believing he was dead. However, Chimera reassembled himself and promised to visit the Wattersons, though he has not appeared since.
Josh
Josh and Anais share an odd friendship.
Josh and Anais first encounter one another in the former's debut episode, "The Guy." As Anais looks for a friend, she finds Josh, who agrees to be friends with her. However, Gumball and Darwin do not trust Josh, and thus attempt to ruin the pair's friendship. Eventually, the two are proven somewhat right, when it is shown that Josh is in fact a conspiracy theorist who follows the anuran god Kratok. It is unclear if he remains friends with Anais, as the two never interact again, but they are not seen to properly have any falling out, and Josh still appears to care about Anais, as he wants to save her with him until the rise of Kratok.
Jodie
Jodie and Anais' friendship was highly unhealthy.
Jodie is a girl in Anais' class. In "The Parasite," Anais becomes obsessed with her, to the point of becoming a social (and then literal) parasite. Anais' brothers, however, are convinced that it is Jodie who is the parasite, and so attempt to remove her from Anais' life, going so far as attempted murder with poisonous sandwiches. However, when it is revealed that Anais is the true parasite, the boys remove them. Anais is guilty, and gives an emotional speech asking Jodie whether they can still be friends. Jodie leaves during the speech, implying that she wants nothing to do with Anais. Their status from this episode on is unclear, due to them never interacting again after this episode.
Friends
Jamie
Jamie and Anais are genuine friends.
Anais' first interaction with Jamie comes in "The Buddy," wherein they are framed for property damage (of the library computers) and must work together to clear their names. They eventually find the true culprit— the librarian herself. They team up and fight the librarian using classic works of fiction and emerge victorious. Anais hopes to be Jamie's 'buddy' at this point, but Jamie declares that they are not buddies, much to Anais' disappointment. However, this is shown to be due to Jamie's stupidity, as she meant that they are not buddies but friends. Delighted, Anais walks with Jamie down the hallway, as Jamie gives her tips on how to act cool.
Unrequited Admirers
Billy
Anais kicks Billy
Anais meets Billy for their playdate, arranged by Nicole in "The Egg." They discover they share a lot of interests, and it is even implied that they fall in love with each other. However their short-lived friendship ends after Billy states that he does not like Daisy the Donkey. In "The Pest," Billy has been bullying Anais at Elmore Junior High, and she vows to beat him up. At the end of the episode, Billy reveals he actually like-likes her and is just angry she doesn't like him back. He realizes that he should leave her alone, but she knocks him into a wall.
Status unclear
Carrie
They have interacted in Halloween.
They have only interacted once with each other in "Halloween" by having Carrie say no to Anais' offer to join the ghost party. Because of this, Anais might not be fond of Carrie. In "The Quest," Carrie and Tobias take away Anais' Daisy the Donkey doll and toss it back and forward.
Tina
Anais does not interact with Tina much. In "The Quest," she and her brothers try taking Daisy the Donkey back from Tina. Later, she lets Tina keep it. In "The Fight," Anais stands up to Tina after she discovers her brother, Gumball, being bullied by her. In the end, she finds that Tina and Gumball have become friends.
Daisy the Donkey
Daisy is dreaming about she torturing a doll of Anais.
Though Daisy is (usually) not alive, Anais loves her very much. This is shown in "The Remote," and especially in "The World" where it reveals that she got Daisy for her birthday. Even in "The Car," Anais begs and later forces her mother to bring her to the "Daisy the Donkey on Ice" show and makes her brothers take Daisy back in "The Quest." It can also be shown in "The Egg", where she lost her interest in Billy after he insulted Daisy. In "The World" though, when Daisy becomes alive and says she loves Anais too, Anais gets scared and kicks her away. However, Anais does not just use Daisy as a doll, but extends it as a place to hide objects as shown in "The Remote." She seem to care for her alot, seen in "The Detective," where she tries to find out who stole Daisy. However, in "The Night," it is shown that these feelings may not be reciprocated, as Daisy's dream involves her torturing a doll of Anais with pleasure, with this pleasure continuing when she wakes up. It is unclear if this scene is intended to mean anything, however.